Product Description
GABARAP Antibody (Ascites) [APG03307G] | Leading Biology
Product Category: Monoclonal Antibodies
Host: Mouse
Species Reactivity: H, M
Specificity: Purified His-tagged GABARAP protein (Fragment) was used to produced this monoclonal antibody.
Cellular Localisation: Endomembrane system {ECO:0000250|UniProtKB:P60517}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P60517}. Golgi apparatus membrane {ECO:0000250|UniProtKB:P60517}. Cytoplasmic vesicle, autophagosome. Cytoplasmic vesicle {ECO:0000250|UniProtKB:P60517}. Note=Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity) Localizes also to discrete punctae along the ciliary axoneme (By similarity) . {ECO:0000250|UniProtKB:P60517, ECO:0000250|UniProtKB:Q9DCD6}
Molecular Weight: 13918
Clone: Monoclonal
Gene Name: GABARAP
Gene ID: 11337
Function: Ubiquitin-like modifier that plays a role in intracellular transport of GABA (A) receptors and its interaction with the cytoskeleton (PubMed:9892355) . Involved in autophagy: while LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:15169837, PubMed:20562859, PubMed:22948227) . Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538) . Also required for the local activition of the CUL3 (KBTBD6/7) E3 ubiquitin ligase complex, regulating ubiquitination and degradation of TIAM1, a guanyl-nucleotide exchange factor (GEF) that activates RAC1 and downstream signal transduction (PubMed:25684205) . Thereby, regulates different biological processes including the organization of the cytoskeleton, cell migration and proliferation (PubMed:25684205) . Involved in apoptosis (PubMed:15977068) .
Summary: Tissue Location: Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
Form: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: WB
Dilution: WB--1:1000 WB--1:500~2000
Synonyms: Gamma-aminobutyric acid receptor-associated protein, GABA (A) receptor-associated protein, MM46, GABARAP, FLC3B