Product Description
ERCC8 Antibody (Center) [APR30706G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H, M
Specificity: This ERCC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210-238 amino acids from the Central region of human ERCC8.
Cellular Localisation: Nucleus. Nucleus matrix. Note=UV-induced translocation to the nuclear matrix is dependent on ERCC6.
Molecular Weight: 44055
Clone: Polyclonal
Gene Name: ERCC8
Gene ID: 1161
Function: Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair. The CSA complex (DCX (ERCC8) complex) promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair. It is required for the recruitment of XAB2, HMGN1 and TCEA1/TFIIS to a transcription-coupled repair complex which removes RNA polymerase II-blocking lesions from the transcribed strand of active genes. Plays a role in DNA single-strand and double- strand breaks (DSSBs) repair; involved in repair of DSSBs by non- homologous end joining (NHEJ) (PubMed:29545921) .
Summary: N/A
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: WB, IHC-P
Dilution: WB--1:1000 IHC-P--1:100~500
Synonyms: DNA excision repair protein ERCC-8, Cockayne syndrome WD repeat protein CSA, ERCC8, CKN1, CSA