Product Description
FBXW8 Antibody (Center) [APR30666G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H, M
Specificity: This FBXW8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-299 amino acids from the Central region of human FBXW8.
Cellular Localisation: Cytoplasm, perinuclear region. Golgi apparatus. Note=Colocalizes with CUL7 at the Golgi apparatus in neurons. {ECO:0000250|UniProtKB:P0DL28}
Molecular Weight: 67394
Clone: Polyclonal
Gene Name: FBXW8
Gene ID: 26259
Function: Substrate-recognition component of a Cul7-RING ubiquitin- protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. The Cul7- RING (FBXW8) complex mediates ubiquitination and consequent degradation of GORASP1, acting as a component of the ubiquitin ligase pathway that regulates Golgi morphogenesis and dendrite patterning in brain (PubMed:21572988) . Mediates ubiquitination and degradation of IRS1 in a mTOR-dependent manner: the Cul7-RING (FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2) (PubMed:18498745) . The Cul7-RING (FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradation, thereby affecting cell proliferation and differentiation (PubMed:24362026) . Associated component of the 3M complex, suggesting that it mediates some of 3M complex functions (PubMed:24793695) .
Summary: N/A
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: WB, IHC-P, FC
Dilution: WB--1:1000 IHC-P--1:100~500 FC--1:10~50
Synonyms: F-box/WD repeat-containing protein 8, F-box and WD-40 domain-containing protein 8, F-box only protein 29, FBXW8, FBW6, FBW8, FBX29, FBXO29, FBXW6