Product Description
FGF2 Antibody (Center) [APR30551G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This FGF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-191 amino acids from the Central region of human FGF2.
Cellular Localisation: Secreted. Nucleus. Note=Exported from cells by an endoplasmic reticulum (ER) /Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane (PubMed:20230531) . Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol (PubMed:22321063) . Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57 (PubMed:22321063)
Molecular Weight: 30770
Clone: Polyclonal
Gene Name: FGF2
Gene ID: 2247
Function: Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:8663044) . Also acts as an integrin ligand which is required for FGF2 signaling (PubMed:28302677) . Binds to integrin ITGAV:ITGB3 (PubMed:28302677) . Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed:8663044, PubMed:28302677) . Functions as a potent mitogen in vitro (PubMed:1721615, PubMed:3964259, PubMed:3732516) . Can induce angiogenesis (PubMed:23469107, PubMed:28302677) . Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed:29501879) .
Summary: Tissue Location: Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: WB, IHC-P, IF
Dilution: WB--1:2000
Synonyms: Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2, HBGF-2, FGF2, FGFB