Product Description
JAM2 Antibody (N-term) [APR03925G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This JAM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-91 amino acids from the N-terminal region of human JAM2.
Cellular Localisation: Cell membrane; Single-pass type I membrane protein. Cell junction. Cell junction, tight junction {ECO:0000250|UniProtKB:Q9JI59}. Note=Localized at tight junctions of both epithelial and endothelial cells (By similarity) . Specifically localized within the somatodendritic compartment of neurons and excluded from the axon (By similarity) {ECO:0000250|UniProtKB:Q9JI59}
Molecular Weight: 33207
Clone: Polyclonal
Gene Name: JAM2 (HGNC:14686)
Gene ID: 58494
Function: Junctional adhesion protein that mediates heterotypic cell- cell interactions with its cognate receptor JAM3 to regulate different cellular processes (PubMed:11590146, PubMed:11823489, PubMed:24357068) . Plays a role in homing and mobilization of hematopoietic stem and progenitor cells within the bone marrow (PubMed:24357068) . At the surface of bone marrow stromal cells, it contributes to the retention of the hematopoietic stem and progenitor cells expressing JAM3 (PubMed:11590146, PubMed:24357068) . Plays a central role in leukocytes extravasation by facilitating not only transmigration but also tethering and rolling of leukocytes along the endothelium (PubMed:12239159) . Tethering and rolling of leukocytes are dependent on the binding by JAM2 of the integrin alpha-4/beta-1 (PubMed:12070135) . Plays a role in spermatogenesis where JAM2 and JAM3, which are respectively expressed by Sertoli and germ cells, mediate an interaction between both cell types and play an essential role in the anchorage of germ cells onto Sertoli cells and the assembly of cell polarity complexes during spermatid differentiation (By similarity) . Also functions as an inhibitory somatodendritic cue that prevents the myelination of non-axonal parts of neurons (By similarity) . During myogenesis, it is involved in myocyte fusion (By similarity) . May also play a role in angiogenesis (By similarity) .
Summary: Tissue Location: Highly expressed in heart, placenta, lung, foreskin and lymph node (PubMed:10779521, PubMed:10945976) . Prominently expressed on high endothelial venules and also present on the endothelia of other vessels (at protein level) (PubMed:10779521, PubMed:10945976) . Also expressed in the brain in the caudate nuclei (PubMed:31851307) .
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: IHC-P, WB
Dilution: WB--1:1000 IHC-P--1:100~500
Synonyms: Junctional adhesion molecule B, JAM-B, Junctional adhesion molecule 2, JAM-2, Vascular endothelial junction-associated molecule, VE-JAM, CD322, JAM2, C21orf43, VEJAM