Product Description
LOXL3 Antibody (C-term) [APR17208G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This LOXL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 715-744 amino acids from the C-terminal region of human LOXL3.
Cellular Localisation: Secreted, extracellular space {ECO:0000250|UniProtKB:Q9Z175}. Cytoplasm. Nucleus Note=It is unclear how LOXL3 is both intracellular (cytoplasmic and nuclear) and extracellular: it contains a clear signal sequence and is predicted to localize in the extracellular medium. However, the intracellular location is clearly reported and at least another protein of the family (LOXL2) also has intracellular and extracellular localization despite the presence of a signal sequence (PubMed:28065600) . [Isoform 2]: Cytoplasm. Secreted, extracellular space
Molecular Weight: 83166
Clone: Polyclonal
Gene Name: LOXL3 {ECO:0000303|PubMed:11386757, ECO:0000312|HGNC:HGNC:13869}
Gene ID: 84695
Function: Protein-lysine 6-oxidase that mediates the oxidation of peptidyl lysine residues to allysine in target proteins (PubMed:17018530, PubMed:28065600) . Catalyzes the post-translational oxidative deamination of peptidyl lysine residues in precursors of elastin and different types of collagens, a prerequisite in the formation of cross-links between collagens and elastin (PubMed:17018530) . Required for somite boundary formation by catalyzing oxidation of fibronectin (FN1), enhancing integrin signaling in myofibers and their adhesion to the myotendinous junction (MTJ) (By similarity) . Acts as a regulator of inflammatory response by inhibiting differentiation of naive CD4 (+) T-cells into T-helper Th17 or regulatory T-cells (Treg) : acts by interacting with STAT3 in the nucleus and catalyzing both deacetylation and oxidation of lysine residues on STAT3, leading to disrupt STAT3 dimerization and inhibit STAT3 transcription activity (PubMed:28065600) . Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated (PubMed:28065600) . Also able to catalyze deacetylation of lysine residues on STAT3 (PubMed:28065600) .
Summary: Tissue Location: Isoform 1: Predominantly detected in the heart, placenta, lung, and small intestine (PubMed:17018530) . Isoform 2: Highly detected in the kidney, pancreas, spleen, and thymus, and is absent in lung (PubMed:17018530) . In eye, present in all layers of corneas as well as in the limbus and conjunctiva (at protein level) (PubMed:26218558) .
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: IHC-P, WB
Dilution: WB--1:2000 IHC-P--1:100~500
Synonyms: Lysyl oxidase homolog 3, 143-, Lysyl oxidase-like protein 3, LOXL3, LOXL