Product Description
EMP2 Antibody (C-term) [APR03839G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: M
Specificity: This EMP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-129 amino acids from the C-terminal region of human EMP2.
Cellular Localisation: Golgi apparatus membrane {ECO:0000250|UniProtKB:O88662}; Multi-pass membrane protein. Cell membrane. Apical cell membrane {ECO:0000250|UniProtKB:O88662}. Membrane raft. Cytoplasm Nucleus {ECO:0000250|UniProtKB:Q66HH2}. Note=Localizes in cytoplasm, foot processes and cell bodies of podocytes and nucleus of endothelial cells of kidney. Localizes to the apical cell surface in the luminal epithelium and glandular epithelium. Colocalized with ITGB1 and GPI- anchor proteins on plasma membrane. {ECO:0000250|UniProtKB:O88662, ECO:0000250|UniProtKB:Q66HH2}
Molecular Weight: 19199
Clone: Polyclonal
Gene Name: EMP2
Gene ID: 2013
Function: Functions as a key regulator of cell membrane composition by regulating proteins surface expression. Also, plays a role in regulation of processes including cell migration, cell proliferation, cell contraction and cell adhesion. Negatively regulates caveolae formation by reducing CAV1 expression and CAV1 amount by increasing lysosomal degradation (PubMed:24814193) . Facilitates surface trafficking and formation of lipid rafts bearing GPI-anchor proteins (By similarity) . Regulates surface expression of MHC1 and ICAM1 proteins increasing susceptibility to T-cell mediated cytotoxicity (By similarity) . Regulates the plasma membrane expression of the integrin heterodimers ITGA6-ITGB1, ITGA5-ITGB3 and ITGA5-ITGB1 resulting in modulation of cell-matrix adhesion (PubMed:16216233) . Also regulates many processes through PTK2. Regulates blood vessel endothelial cell migration and angiogenesis by regulating VEGF protein expression through PTK2 activation (PubMed:23439602) . Regulates cell migration and cell contraction through PTK2 and SRC activation (PubMed:21637765, PubMed:22728127) . Regulates focal adhesion density, F-actin conformation and cell adhesion capacity through interaction with PTK2 (PubMed:19494199) . Positively regulates cell proliferation (PubMed:24814193) . Plays a role during cell death and cell blebbing (PubMed:12107182) . Promotes angiogenesis and vasculogenesis through induction of VEGFA via a HIF1A-dependent pathway (PubMed:23334331) . Also plays a role in embryo implantation by regulating surface trafficking of integrin heterodimer ITGA5-ITGB3 (PubMed:16487956) . May play a role in glomerular filtration (By similarity) .
Summary: Tissue Location: Expressed in ciliary body epithelia, sclera, cornea, and retinal pigment epithelium (at protein level) (PubMed:12710941) .
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: WB, IHC-P
Dilution: WB--1:1000 IHC-P--1:100~500
Synonyms: Epithelial membrane protein 2, EMP-2, Protein XMP, EMP2, XMP