Product Description
EZH1 Antibody (Center) [APR31969G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This EZH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-422 amino acids from the Central region of human EZH1.
Cellular Localisation: Nucleus. Note=Colocalizes with trimethylated 'Lys-27' of histone H3
Molecular Weight: 85271
Clone: Polyclonal
Gene Name: EZH1
Gene ID: 2145
Function: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
Summary: N/A
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: WB, IHC-P
Dilution: WB--1:1000 IHC-P--1:100~500
Synonyms: Histone-lysine N-methyltransferase EZH1, ENX-2, Enhancer of zeste homolog 1, EZH1, KIAA0388