Product Description
FBXL2 Antibody (C-term) [APR32113G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This FBXL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-395 amino acids from the C-terminal region of human FBXL2.
Cellular Localisation: Membrane; Lipid- anchor
Molecular Weight: 47062
Clone: Polyclonal
Gene Name: FBXL2 (HGNC:13598)
Gene ID: 25827
Function: Calcium-activated substrate recognition component of the SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex, SCF (FBXL2), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 does not seem to target phosphodegron within its substrates but rather calmodulin-binding motifs and is thereby antagonized by calmodulin. This is the case for the cyclins CCND2 and CCND3 which polyubiquitination and subsequent degradation are inhibited by calmodulin. Through CCND2 and CCND3 degradation induces cell-cycle arrest in G (0) (PubMed:22020328, PubMed:22323446) . SCF (FBXL2) also mediates PIK3R2 ubiquitination and proteasomal degradation thereby regulating phosphatidylinositol 3-kinase signaling and autophagy (PubMed:23604317) . PCYT1A monoubiquitination by SCF (FBXL2) and subsequent degradation regulates synthesis of phosphatidylcholine, which is utilized for formation of membranes and of pulmonary surfactant (By similarity) .
Summary: Tissue Location: Expressed in brain, heart, kidney, liver, lung, pancreas and placenta.
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: IHC-P, FC, WB
Dilution: WB--1:1000-1:2000 IHC-P--1:100~500 FC--1:10~50
Synonyms: F-box/LRR-repeat protein 2, F-box and leucine-rich repeat protein 2 {ECO:0000312|HGNC:HGNC:13598}, F-box protein FBL2/FBL3 {ECO:0000303|PubMed:10945468, ECO:0000312|EMBL:AAF045101}, FBXL2 (HGNC:13598)