Product Description
NLRP6 Antibody (N-term) [APR31129G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This NLRP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-197 amino acids from the N-terminal region of human NLRP6.
Cellular Localisation: Cytoplasm. Inflammasome. Cell membrane {ECO:0000250|UniProtKB:Q63035}. Nucleus membrane {ECO:0000250|UniProtKB:Q63035}
Molecular Weight: 98768
Clone: Polyclonal
Gene Name: NLRP6
Gene ID: 171389
Function: As the sensor component of the NLRP6 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP6, PYCARD and CASP1 (and possibly CASP4 and CASP5) . Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. The precise NLRP6 activation stimulus has not been identified yet (By similarity) (PubMed:12387869) . Essential for gut mucosal self-renewal and proliferation. Maintains intestinal homeostasis and a healthy intestinal microbiota. This function is, at least partially, mediated by IL18, and not IL1B, produced by nonhematopoietic cells. Influences intestinal barrier function and microbial homeostasis through the regulation of goblet cell mucus secretion. Acts by promoting autophagy in goblet cells, an essential step for mucus granule exocytosis. Its role in goblet cell physiology is inflammasome-dependent, but IL1B- and IL18-independent. During systemic bacterial infections, may negatively regulate inflammatory signaling and inhibit the influx of monocytes and neutrophils to the circulation and to the peritoneum. May promote peripheral nerve recovery following injury via an inflammasome- independent mechanism (By similarity) .
Summary: Tissue Location: Expressed in peripheral blood leukocytes, predominantly in granulocytes and, at lower levels, in CD4 (+) and CD8 (+) T-cells. Expressed in colonic myofibroblasts (at protein level) (PubMed:21593405) .
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: WB
Dilution: WB--1:2000
Synonyms: NACHT, LRR and PYD domains-containing protein 6, Angiotensin II/vasopressin receptor, PYRIN-containing APAF1-like protein 5, NLRP6, NALP6, PYPAF5