Product Description
POLK Antibody (Center) [APR31899G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This POLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 531-560 amino acids from the Central region of human POLK.
Cellular Localisation: Nucleus. Note=Detected throughout the nucleus and at replication foci (PubMed:12414988) . Recruited to DNA damage sites in response to ultraviolet irradiation: N6-methyladenosine (m6A) - containing mRNAs accumulate in the vicinity of DNA damage sites and their presence is required to recruit POLK (PubMed:28297716)
Molecular Weight: 98809
Clone: Polyclonal
Gene Name: POLK
Gene ID: 51426
Function: DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
Summary: Tissue Location: Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: WB
Dilution: WB--1:1000
Synonyms: DNA polymerase kappa, DINB protein, DINP, POLK, DINB1