Product Description
RFTN1 Antibody (Center) [APR32792G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This RFTN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-198 amino acids from the Central region of human RFTN1.
Cellular Localisation: Cell membrane; Lipid-anchor. Cytoplasm. Membrane raft. Endosome. Early endosome. Note=Translocates from cytoplasm to cell membrane where it colocalizes with poly (I:C) and then moves to endosomes where it colocalizes with TLR3 (PubMed:21266579) Translocates from cytoplasm to cell membrane where it colocalizes with TLR4 and then together with TLR4 moves to endosomes, upon lipopolysaccharide stimulation (PubMed:27022195)
Molecular Weight: 63146
Clone: Polyclonal
Gene Name: RFTN1
Gene ID: 23180
Function: Involved in protein trafficking via association with clathrin and AP2 complex (PubMed:27022195, PubMed:21266579) . Upon bacterial lipopolysaccharide stimulation, mediates internalization of TLR4 to endosomes in dendritic cells and macrophages; and internalization of poly (I:C) to TLR3-positive endosomes in myeloid dendritic cells and epithelial cells; resulting in activation of TICAM1-mediated signaling and subsequent IFNB1 production (PubMed:27022195, PubMed:21266579) . Involved in T-cell antigen receptor-mediated signaling by regulating tyrosine kinase LCK localization, T-cell dependent antibody production and cytokine secretion (By similarity) . May regulate B-cell antigen receptor-mediated signaling (PubMed:12805216) . May play a pivotal role in the formation and/or maintenance of lipid rafts (PubMed:12805216) .
Summary: Tissue Location: Expressed in B-cells (at protein level) (PubMed:12805216) . Expressed in dendritic cells and macrophages (PubMed:27022195, PubMed:21266579) .
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Application: WB
Dilution: WB--1:1000
Synonyms: Raftlin, Cell migration-inducing gene 2 protein, Raft-linking protein, RFTN1, KIAA0084