Product Description
SMYD3 Antibody (N-term) [APR32422G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This SMYD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-35 amino acids from the N-terminal region of human SMYD3.
Cellular Localisation: Cytoplasm. Nucleus. Note=Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.
Molecular Weight: 49097
Clone: Polyclonal
Gene Name: SMYD3
Gene ID: 64754
Function: Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation (PubMed:15235609, PubMed:22419068) . Also methylates 'Lys-5' of histone H4 (PubMed:22419068) . Plays an important role in transcriptional activation as a member of an RNA polymerase complex (PubMed:15235609) . Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences (PubMed:15235609) .
Summary: Tissue Location: Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: WB, IHC-P, FC
Dilution: WB--1:1000 IHC-P--1:100~500 FC--1:10~50
Synonyms: Histone-lysine N-methyltransferase SMYD3, SET and MYND domain-containing protein 3, Zinc finger MYND domain-containing protein 1, SMYD3, ZMYND1, ZNFN3A1