Product Description
Bcl-2 Antibody (Center Ser70) [APG02223G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This Bcl-2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-76 amino acids from the Central region of human Bcl-2.
Cellular Localisation: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein
Molecular Weight: 26266
Clone: Polyclonal
Gene Name: BCL2
Gene ID: 596
Function: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370) . Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354) . Appears to function in a feedback loop system with caspases (PubMed:11368354) . Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354) . Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:21358617, PubMed:20889974) . May attenuate inflammation by impairing NLRP1- inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785) .
Summary: Tissue Location: Expressed in a variety of tissues.
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Application: WB
Dilution: WB--1:1000
Synonyms: Apoptosis regulator Bcl-2, BCL2