Product Description
FTO Antibody (N-term) [AMM04628G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This FTO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human FTO.
Cellular Localisation: Nucleus. Nucleus speckle. Cytoplasm Note=Localizes mainly in the nucleus, where it is able to demethylate N (6) -methyladenosine (m6A) and N (6), 2'-O-dimethyladenosine cap (m6A (m) ) in U6 small nuclear RNA (snRNA), N (1) -methyladenine from tRNAs and internal m6A in mRNAs (PubMed:30197295) . In the cytoplasm, mediates demethylation of m6A and m6A (m) in mRNAs and N (1) -methyladenine from tRNAs (PubMed:30197295) .
Molecular Weight: 58282
Clone: Polyclonal
Gene Name: FTO {ECO:0000303|PubMed:17496892, ECO:0000312|HGNC:HGNC:24678}
Gene ID: 79068
Function: RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis (PubMed:22002720, PubMed:26458103, PubMed:28002401, PubMed:30197295, PubMed:26457839, PubMed:25452335) . Specifically demethylates N (6) - methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:22002720, PubMed:26458103, PubMed:30197295, PubMed:26457839, PubMed:25452335) . M6A demethylation by FTO affects mRNA expression and stability (PubMed:30197295) . Also able to demethylate m6A in U6 small nuclear RNA (snRNA) (PubMed:30197295) . Mediates demethylation of N (6), 2'-O- dimethyladenosine cap (m6A (m) ), by demethylating the N (6) - methyladenosine at the second transcribed position of mRNAs and U6 snRNA (PubMed:28002401, PubMed:30197295) . Demethylation of m6A (m) in the 5'-cap by FTO affects mRNA stability by promoting susceptibility to decapping (PubMed:28002401) . Also acts as a tRNA demethylase by removing N (1) -methyladenine from various tRNAs (PubMed:30197295) . Has no activity towards 1-methylguanine (PubMed:20376003) . Has no detectable activity towards double-stranded DNA (PubMed:20376003) . Also able to repair alkylated DNA and RNA by oxidative demethylation: demethylates single-stranded RNA containing 3-methyluracil, single- stranded DNA containing 3-methylthymine and has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3- methylcytosine (PubMed:18775698, PubMed:20376003) . Ability to repair alkylated DNA and RNA is however unsure in vivo (PubMed:18775698, PubMed:20376003) . Involved in the regulation of fat mass, adipogenesis and body weight, thereby contributing to the regulation of body size and body fat accumulation (PubMed:18775698, PubMed:20376003) . Involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells (PubMed:26287746) . Regulates activity of the dopaminergic midbrain circuitry via its ability to demethylate m6A in mRNAs (By similarity) . Plays an oncogenic role in a number of acute myeloid leukemias by enhancing leukemic oncogene-mediated cell transformation: acts by mediating m6A demethylation of target transcripts such as MYC, CEBPA, ASB2 and RARA, leading to promote their expression (PubMed:28017614, PubMed:29249359) .
Summary: Tissue Location: Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary (PubMed:17434869, PubMed:17496892) . Highly expressed in highly expressed in acute myeloid leukemias (AML) with t (11;11) (q23;23) with KMT2A/MLL1 rearrangements, t (15;17) (q21;q21) /PML-RARA, FLT3-ITD, and/or NPM1 mutations (PubMed:28017614) .
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: WB, IHC-P, FC
Dilution: WB--1:1000 IHC-P--1:100~500 FC--1:10~50
Synonyms: Alpha-ketoglutarate-dependent dioxygenase FTO, 11411-, Fat mass and obesity-associated protein, FTO, KIAA1752