Product Description
GRN Antibody (C-term) [APR04581G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This GRN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 563-591 amino acids from the C-terminal region of human GRN.
Cellular Localisation: Secreted. Lysosome Note=Endocytosed by SORT1 and delivred to lysosomes (PubMed:21092856, PubMed:28073925) . Targeted to lysosome by PSAP via M6PR and LRP1, in both biosynthetic and endocytic pathways (PubMed:26370502, PubMed:28073925) . Co-localized with GBA in the intracellular trafficking compartments until to lysosome (By similarity) {ECO:0000250|UniProtKB:P28798, ECO:0000269|PubMed:21092856, ECO:0000269|PubMed:26370502, ECO:0000269|PubMed:28073925}
Molecular Weight: 63544
Clone: Polyclonal
Gene Name: GRN (HGNC:4601)
Gene ID: 2896
Function: Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation (PubMed:28541286, PubMed:28073925, PubMed:18378771, PubMed:28453791, PubMed:12526812) . Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes (PubMed:28453791, PubMed:28541286) . Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB (PubMed:28073925) . In addition, functions as wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures (By similarity) . Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases (PubMed:12526812) . Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity (PubMed:18378771) .
Summary: Tissue Location: In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Application: WB, IHC-P
Dilution: IHC-P--1:100~500 WB--1:1000
Synonyms: Granulins, Proepithelin, PEPI, Acrogranin, Glycoprotein of 88 Kda, Progranulin, Paragranulin, Granulin-1, Granulin G, Granulin-2, Granulin F, Granulin-3, Granulin B, Granulin-4, Granulin A, Granulin-5, Granulin C, Granulin-6, Granulin D, Granulin-7, Granulin E, GRN