Product Description
HK1 Antibody (C-term) [APR07758G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This HK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 705-734 amino acids from the C-terminal region of human HK1.
Cellular Localisation: Mitochondrion outer membrane; Peripheral membrane protein. Cytoplasm, cytosol. Note=The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane (Probable) . Dissociates from the mitochondrial outer membrane following inhibition by N-acetyl-D-glucosamine, leading to relocation to the cytosol (PubMed:27374331) .
Molecular Weight: 102486
Clone: Polyclonal
Gene Name: HK1 (HGNC:4922)
Gene ID: 3098
Function: Catalyzes the phosphorylation of various hexoses, such as D- glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6- phosphate, respectively) (PubMed:1637300, PubMed:25316723, PubMed:27374331) . Does not phosphorylate N-acetyl-D-glucosamine (PubMed:27374331) . Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity) . Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan (PubMed:27374331) . When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331) .
Summary: Tissue Location: Isoform 2: Erythrocyte specific (Ref.6) . Isoform 3: Testis-specific (PubMed:10978502) . Isoform 4: Testis-specific (PubMed:10978502) . {ECO:0000269|PubMed:10978502, ECO:0000269|Ref.6}
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: WB, IHC-P
Dilution: WB--1:1000 IHC-P--1:100~500
Synonyms: Hexokinase-1, Brain form hexokinase, Hexokinase type I, HK I, HK1