Product Description
PIP5K3 Antibody (Center) [APR10846G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This PIP5K3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1531-1561 amino acids from the Central region of human PIP5K3.
Cellular Localisation: Endosome membrane; Peripheral membrane protein {ECO:0000250|UniProtKB:Q9Z1T6}. Early endosome membrane; Peripheral membrane protein. Cytoplasmic vesicle, phagosome membrane; Peripheral membrane protein. Late endosome membrane; Peripheral membrane protein {ECO:0000250|UniProtKB:Q9Z1T6}. Note=Mainly associated with membranes of the late endocytic pathway.
Molecular Weight: 237136
Clone: Polyclonal
Gene Name: PIKFYVE (HGNC:23785)
Gene ID: 200576
Function: Dual specificity kinase implicated in myriad essential cellular processes such as maintenance of endomembrane homeostasis, and endocytic-vacuolar pathway, lysosomal trafficking, nuclear transport, stress- or hormone-induced signaling and cell cycle progression (PubMed:23086417) . The PI (3, 5) P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3, 5-bisphosphate (PtdIns (3, 5) P2) . Sole enzyme to catalyze the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo- inositol ring, to form (PtdIns (3, 5) P2) (PubMed:17556371) . Also catalyzes the phosphorylation of phosphatidylinositol on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 5- phosphate (PtdIns (5) P) (PubMed:22621786) . Has serine-protein kinase activity and is able to autophosphorylate and transphosphorylate. Autophosphorylation inhibits its own phosphatidylinositol 3-phosphate 5-kinase activity, stimulates FIG4 lipid phosphatase activity and downregulates lipid product formation (PubMed:33098764) . Involved in key endosome operations such as fission and fusion in the course of endosomal cargo transport (PubMed:22621786) . Required for the maturation of early into late endosomes, phagosomes and lysosomes (PubMed:30612035) . Regulates vacuole maturation and nutrient recovery following engulfment of macromolecules, initiates the redistribution of accumulated lysosomal contents back into the endosome network (PubMed:27623384) . Critical regulator of the morphology, degradative activity, and protein turnover of the endolysosomal system in macrophages and platelets (By similarity) . In neutrophils, critical to perform chemotaxis, generate ROS, and undertake phagosome fusion with lysosomes (PubMed:28779020) . Plays a key role in the processing and presentation of antigens by major histocompatibility complex class II (MHC class II) mediated by CTSS (PubMed:30612035) . Regulates melanosome biogenesis by controlling the delivery of proteins from the endosomal compartment to the melanosome (PubMed:29584722) . Essential for systemic glucose homeostasis, mediates insulin-induced signals for endosome/actin remodeling in the course of GLUT4 translocation/glucose uptake activation (By similarity) . Supports microtubule-based endosome- to-trans-Golgi network cargo transport, through association with SPAG9 and RABEPK (By similarity) . Mediates EGFR trafficking to the nucleus (PubMed:17909029) .
Summary: N/A
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: WB, IHC-P
Dilution: WB--1:1000 IHC-P--1:100~500
Synonyms: 1-phosphatidylinositol 3-phosphate 5-kinase, Phosphatidylinositol 3-phosphate 5-kinase, FYVE finger-containing phosphoinositide kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase type III, PIPkin-III, Type III PIP kinase, PIKFYVE, KIAA0981, PIP5K3