Product Description
SIRT7 Antibody (C-term) [APR07041G] | Leading Biology
Product Category: Polyclonal Antibodies
Host: Rabbit
Species Reactivity: H
Specificity: This SIRT7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-360 amino acids from the C-terminal region of human SIRT7.
Cellular Localisation: Nucleus, nucleolus. Nucleus, nucleoplasm. Chromosome. Cytoplasm. Note=Mainly localizes in the nucleolus and nucleoplasm (PubMed:24207024, PubMed:28886238, PubMed:28790157, PubMed:31075303) . Associated with rDNA promoter and transcribed region (PubMed:16079181, PubMed:19174463) . Associated with nucleolar organizer regions during mitosis (PubMed:16079181, PubMed:19174463) . In response to stress, released from nucleolus to nucleoplasm (PubMed:24207024) Associated with chromatin (PubMed:22722849) . In response to DNA damage, recruited to DNA double-strand breaks (DSBs) sites (PubMed:27436229) (Probable) . Located close to the nuclear membrane when in the cytoplasm (PubMed:11953824) .
Molecular Weight: 44898
Clone: Polyclonal
Gene Name: SIRT7 {ECO:0000303|PubMed:22722849, ECO:0000312|HGNC:HGNC:14935}
Gene ID: 51547
Function: NAD-dependent protein-lysine deacylase that can act both as a deacetylase or deacylase (desuccinylase, depropionylase and deglutarylase), depending on the context (PubMed:22722849, PubMed:26907567, PubMed:30653310, PubMed:31542297) . Specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac) (PubMed:22722849, PubMed:30420520) . In contrast to other histone deacetylases, displays strong preference for a specific histone mark, H3K18Ac, directly linked to control of gene expression (PubMed:22722849, PubMed:30653310) . H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors; SIRT7 thereby acts as a transcription repressor (PubMed:22722849) . Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells (PubMed:22722849) . Also able to mediate deacetylation of histone H3 at 'Lys-36' (H3K36Ac) in the context of nucleosomes (PubMed:30653310) . Also mediates deacetylation of non-histone proteins, such as ATM, CDK9, DDX21, DDB1, FBL, FKBP5/FKBP51, GABPB1, RAN, RRP9/U3-55K and POLR1E/PAF53 (PubMed:24207024, PubMed:26867678, PubMed:28147277, PubMed:28886238, PubMed:28426094, PubMed:30540930, PubMed:31075303, PubMed:30944854, PubMed:28790157) . Enriched in nucleolus where it stimulates transcription activity of the RNA polymerase I complex (PubMed:16618798, PubMed:19174463, PubMed:24207024) . Acts by mediating the deacetylation of the RNA polymerase I subunit POLR1E/PAF53, thereby promoting the association of RNA polymerase I with the rDNA promoter region and coding region (PubMed:16618798, PubMed:19174463, PubMed:24207024) . In response to metabolic stress, SIRT7 is released from nucleoli leading to hyperacetylation of POLR1E/PAF53 and decreased RNA polymerase I transcription (PubMed:24207024) . Required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis (PubMed:19174463) . Promotes pre-ribosomal RNA (pre-rRNA) cleavage at the 5'-terminal processing site by mediating deacetylation of RRP9/U3- 55K, a core subunit of the U3 snoRNP complex (PubMed:26867678) . Mediates 'Lys-37' deacetylation of Ran, thereby regulating the nuclear export of NF-kappa-B subunit RELA/p65 (PubMed:31075303) . Acts as a regulator of DNA damage repair by mediating deacetylation of ATM during the late stages of DNA damage response, promoting ATM dephosphorylation and deactivation (PubMed:30944854) . Suppresses the activity of the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes by mediating deacetylation of DDB1, which prevents the interaction between DDB1 and CUL4 (CUL4A or CUL4B) (PubMed:28886238) . Activates RNA polymerase II transcription by mediating deacetylation of CDK9, thereby promoting 'Ser-2' phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (PubMed:28426094) . Deacetylates FBL, promoting histone- glutamine methyltransferase activity of FBL (PubMed:30540930) . Acts as a regulator of mitochondrial function by catalyzing deacetylation of GABPB1 (By similarity) . Regulates Akt/AKT1 activity by mediating deacetylation of FKBP5/FKBP51 (PubMed:28147277) . Required to prevent R- loop-associated DNA damage and transcription-associated genomic instability by mediating deacetylation and subsequent activation of DDX21, thereby overcoming R-loop-mediated stalling of RNA polymerases (PubMed:28790157) . In addition to protein deacetylase activity, also acts as protein-lysine deacylase (PubMed:27436229, PubMed:27997115, PubMed:31542297) . Acts as a protein depropionylase by mediating depropionylation of Osterix (SP7), thereby regulating bone formation by osteoblasts (By similarity) . Acts as a histone deglutarylase by mediating deglutarylation of histone H4 on 'Lys-91' (H4K91glu) ; a mark that destabilizes nucleosomes by promoting dissociation of the H2A-H2B dimers from nucleosomes (PubMed:31542297) . Acts as a histone desuccinylase: in response to DNA damage, recruited to DNA double- strand breaks (DSBs) and catalyzes desuccinylation of histone H3 on 'Lys-122' (H3K122succ), thereby promoting chromatin condensation and DSB repair (PubMed:27436229) . Also promotes DSB repair by promoting H3K18Ac deacetylation, regulating non-homologous end joining (NHEJ) (By similarity) . Along with its role in DNA repair, required for chromosome synapsis during prophase I of female meiosis by catalyzing H3K18Ac deacetylation (By similarity) . Involved in transcriptional repression of LINE-1 retrotransposon via H3K18Ac deacetylation, and promotes their association with the nuclear lamina (PubMed:31226208) . Required to stabilize ribosomal DNA (rDNA) heterochromatin and prevent cellular senescence induced by rDNA instability (PubMed:29728458) . Acts as a negative regulator of SIRT1 by preventing autodeacetylation of SIRT1, restricting SIRT1 deacetylase activity (By similarity) .
Summary: N/A
Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application: WB, IHC-P
Dilution: IHC-P--1:100~500 WB--1:2000 WB--1:1000
Synonyms: NAD-dependent protein deacetylase sirtuin-7, 351-, Regulatory protein SIR2 homolog 7, SIR2-like protein 7, SIRT7, SIR2L7