Product Description
CBX5 Antibody | 45-372 | ProSci
Host: Goat
Reactivity: Human, Mouse
Homology: Expected Species Reactivity based on sequence homology: Dog
Immunogen: The immunogen for this antibody is: C-NKRKSNFSNSADDIK
Research Area: Cancer
Tested Application: E, WB, IHC, Flow
Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 24-25kDa band observed in lysates of cell lines K562, MCF7 and NIH3T3 (calculated MW of 22.2kDa according to Human NP_036249.1 and Mouse NP_001070257.1) . Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Uterus shows nuclear staining in the epithelial cells of the endometrium. Recommended concentration: 2-4ug/ml.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Specificiy: Variants (NP_036249.1; NP_001120793.1; NP_001120794.1) encode the same protein.
Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: CBX5, HP1-ALPHA, chromobox homolog 5 (HP1 alpha homolog, Drosophila) , HGNC:1555, HP1, HP1Hs-alpha, chromobox homolog 5 (Drosophila HP1 alpha) , Heterochromatin protein-1, HP1A, antigen p25, heterochromatin protein 1 homolog alpha, heterochromatin protein 1-alpha
User Note: N/A
BACKGROUND: N/A