Product Description
PTCH Antibody | 46-246 | ProSci
Host: Goat
Reactivity: Human, Mouse
Homology: Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen: The immunogen for this antibody is: C-HPESRHHPPSNPRQQ
Research Area: Cancer
Tested Application: E, IF, WB
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 150kDa band observed in Human Brain lysates (calculated MW of 153.9kDa according to NP_001077071.1) . Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Immunofluorescence :Expression of the protein seen in the Golgi/membrane of NIH3T3 cells. Recommended concentration: 5ug/ml.
Specificiy: This antibody is expected to recognise all four reported isoforms (NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077075.1) . Reported variants represent identical protein (NP_001077075.1; NP_001077076.1; NP_001077073.1; NP_001077074.1) .
Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: PTCH, patched homolog (Drosophila) , RP11-435O5.3, BCNS, FLJ42602, HPE7, NBCCS, PTC, PTC1, PTCH1, PTCH protein +12b, PTCH protein +4', PTCH protein -10, patched, patched (Drosophila) homolog
User Note: N/A
BACKGROUND: N/A