Product Description
Radixin Antibody | 45-137 | ProSci
Host: Goat
Reactivity: Human, Mouse, Rat
Homology: Expected Species Reactivity based on sequence homology: Dog
Immunogen: The immunogen for this antibody is: C-QARDETKKTQND
Research Area: Signal Transduction
Tested Application: E, WB, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 80kDa band observed in Human Lymph Node, Tonsil and Spleen lysates (calculated MW of 68.6kDa according to NP_002897.1) . The same 80kDa was also observed in lysates of several Mouse and Rat tissues and in lysates of cell lines NIH3T3, HeLa, Jurkat, and A431. The observed molecular weight corresponds to earlier findings in literature (Magendantz et al, J Biol Chem. 1995 Oct 27;270 (43) :25324-7. PMID: 7592691) . Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Placenta and Kidney. Recommended concentration: 2.5ug/ml.
Specificiy: N/A
Positive Control 1: Cat. No. 1315 - Human Tonsil Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: RDX, radixin
User Note: N/A
BACKGROUND: N/A