Product Description
SNAP25 Antibody | 45-149 | ProSci
Host: Goat
Reactivity: Human, Mouse, Rat
Homology: Expected Species Reactivity based on sequence homology: Dog
Immunogen: The immunogen for this antibody is: C-DEANQRATKMLGSG
Research Area: Homeostasis, Neuroscience
Tested Application: E, WB, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 26kDa band observed in Human Cerebellum and in Mouse and Rat Brain lysates (calculated MW of 23.3kDa according to Human NP_003072.2, Mouse NP_035558.1 and Rat NP_001257504.1) . Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Cerebellum shows strong axon staining, particularly in the granular region. Recommended concentration: 1-2ug/ml.
Specificiy: This antibody is expected to recognise both reported isoforms NP_003072.2 (SNAP25A) and NP_570824.1 (SNAP25B) . Reported variants represent identical protein: NP_001309831.1, NP_003072.2 Reported variants represent identical protein: NP_001309834.1, NP_001309832.1, NP_001309833.1, NP_001309839.1, NP_001309836.1, NP_570824.1, NP_001309838.1, NP_001309835.1, NP_001309837.1
Positive Control 1: Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: SNAP25, synaptosomal-associated protein, 25kDa, HGNC:11132, FLJ23079, SNAP, SNAP-25, bA416N4.2, dJ1068F16.2, synaptosomal-associated protein 25, RIC-4, RIC4, SEC9, resistance to inhibitors of cholinesterase 4 homolog, synaptosomal-associated protein 25
User Note: N/A
BACKGROUND: N/A