Product Description
TAK1 Antibody | 46-469 | ProSci
Host: Goat
Reactivity: Human, Mouse
Homology: Expected Species Reactivity based on sequence homology: Rat, Cow
Immunogen: The immunogen for this antibody is: C-AELDQDEKDQQNT
Research Area: Cancer
Tested Application: E, WB, Flow
Application: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 75kDa band observed in lysates of cell lines HeLa, Daudi and NIH3T3, and approx. 80kDa in lysates of cell line U937 (calculated MW of 67.2 according to Human NP_663304.1 and Mouse NP_033342.1) . The observed molecular weight corresponds to different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Specificiy: This antibody is expected to recognise isoform A (NP_003179.1) and isoform B (NP_663304.1) .
Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: MAP3K7, TAK1, mitogen-activated protein kinase kinase kinase 7, TGF1a, TGF-beta activated kinase 1, transforming growth factor-beta-activated kinase 1
User Note: N/A
BACKGROUND: N/A