Product Description
ALS2CR2 Antibody | 45-246 | ProSci
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen: The immunogen for this antibody is: CDFPDEKDSYWEF
Research Area: Cancer
Tested Application: E, WB, IHC, IF
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 35kDa band observed in Human Heart, Liver and Muscle lysates (calculated MW of 47.0kDa according to NP_061041.2 (alpha isoform) and 31.2kDa according to AAF71042.1 (beta isoform) . In transfected HEK293 transiently expressing ILPIP a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Heart shows staining of myocardial fibres in transverse section. Recommended concentration: 3-5ug/ml.Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U251 cells. Recommended concentration: 10ug/ml.
Specificiy: This is expected to recognise both human isoforms: ILPIP-alpha (NP_061041.2) and ILPIP-beta (AAF71042.1) .
Positive Control 1: Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: STRADB, ALS2CR2, ILPIP, PAPK, ILPIPA, CALS-21, PRO1038, amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2, ILP-interacting protein ILPIPA, STE20-related kinase adaptor beta, STRAD beta, pseudokinase ALS2CR2
User Note: N/A
BACKGROUND: N/A