Product Description
DKC1 Antibody | 45-483 | ProSci
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse
Immunogen: The immunogen for this antibody is: C-KRKRESESESDETPP
Research Area: Cancer
Tested Application: E, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 70-75kDa band in Human Bone Marrow, Duodenum and Skin lysates after 0.01ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57.7kDa according to NP_001354.1. The 70-75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Spleen shows strong nuclear staining of select splenocytes. Recommended concentration, 5-10ug/ml.
Specificiy: N/A
Positive Control 1: Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: DKC1, dyskeratosis congenita 1, dyskerin, DKC, NAP57, NOLA4, XAP101,
User Note: N/A
BACKGROUND: N/A