Product Description
Muc5b Antibody | 42-328 | ProSci
Host: Goat
Reactivity: N/A
Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen: The immunogen for this antibody is: CEKEDSSTCQ
Research Area: Signal Transduction
Tested Application: E
Application: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave bands at approx 100kDa and 55kDa in Mouse Lung lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 515kDa according to Mouse NP_083077.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: Muc5b, mucin 5, subtype B, tracheobronchial, 2300002I04Rik, A130042M24, AV085033, MUC5, MUC9, mucin 5b
User Note: N/A
BACKGROUND: N/A