Product Description
NF45 Antibody | 46-677 | ProSci
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen: The immunogen for this antibody is: C-EKAYEKPPEKKEG
Research Area: Immunology
Tested Application: E, WB
Application: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 43kDa band observed in Human Tonsil and Thymus lysates (calculated MW of 43.1kDa according to NP_004506.2) . Recommended concentration: 0.03-0.1ug/ml. This antibody has been successfully used in WB in the following papers: Ye J et al, RNA. 2017 Aug;23 (8) :1270-1284. PMID: 28487382. And Shamanna RA et al, Oncogene. 2013 Oct 24;32 (43) :5176-85 PMID: 23208500.Immunoprecipitation: This antibody was confirmed by a customer in a special IP procedure called PAR-CLIP (see for method PMID: 20644507) . This data is from a previous batch, not on sale.
Specificiy: This antibody is expected to recognize both reported isoforms (NP_004506.2; NP_001254738.1) .
Positive Control 1: Cat. No. 1315 - Human Tonsil Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: ILF2, NF45, interleukin enhancer binding factor 2, 45kDa, MGC8391, PRO3063, interleukin enhancer binding factor 2, nuclear factor of activated T-cells, 45-kDa
User Note: N/A
BACKGROUND: N/A