Product Description
ATP5J Antibody | 19-147 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human ATP5J (NP_001676.2) .
Research Area: Cancer, Neuroscience, Signal Transduction
Tested Application: WB, IF
Application: WB: 1:200 - 1:2000
IF: 1:50 - 1:200
Specificiy: N/A
Positive Control 1: SW480
Positive Control 2: THP-1
Positive Control 3: NCI-H460
Positive Control 4: 22Rv1
Positive Control 5: Mouse kidney
Positive Control 6: Mouse heart
Molecular Weight: Observed: 13kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: ATP synthase-coupling factor 6, mitochondrial, ATPase subunit F6, ATP5J, ATP5A, ATPM
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo complex has nine subunits (a, b, c, d, e, f, g, F6 and 8) . This gene encodes the F6 subunit of the Fo complex. The F6 subunit is required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has 1 or more pseudogenes.