Product Description
CYB5R3 Antibody | 23-012 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 125-334 of human CYB5R3 (NP_001165131.1) .
Research Area: Cancer, Signal Transduction
Tested Application: WB
Application: WB: 1:500 - 1:2000
Specificiy: N/A
Positive Control 1: HepG2
Positive Control 2: HeLa
Positive Control 3: HT-1080
Positive Control 4: NIH/3T3
Positive Control 5: Mouse kidney
Positive Control 6: Mouse brain
Molecular Weight: Observed: 34-36kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: OTTHUMP00000198665, OTTHUMP00000198574, NADH-cytochrome b5 reductase 3 soluble form, NADH-cytochrome b5 reductase 3 membrane-bound form, NADH-cytochrome b5 reductase 3, DIA1, B5R, cytochrome b5 reductase 3, CYB5R3
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias.