Product Description
GPT Antibody | 18-825 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human GPT (NP_005300.1) .
Research Area: Signal Transduction
Tested Application: WB, IHC
Application: WB: 1:200 - 1:1000
IHC: 1:50 - 1:100
Specificiy: N/A
Positive Control 1: Mouse liver
Positive Control 2: Mouse lung
Positive Control 3: Mouse intestine
Positive Control 4: Rat liver
Positive Control 5: Rat heart
Positive Control 6: N/A
Molecular Weight: Observed: 55kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: GPT, AAT1, ALT1, GPT1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: This gene encodes cytosolic alanine aminotransaminase 1 (ALT1) ; also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.