Product Description
IDH2 Antibody | 22-769 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 193-452 of human IDH2 (NP_002159.2) .
Research Area: Cancer, Signal Transduction
Tested Application: WB, IHC, IF, IP, ChIP
Application: WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:20 - 1:50
IP: 1:50 - 1:200
ChIP: 1:20 - 1:100
Specificiy: N/A
Positive Control 1: HL-60
Positive Control 2: MCF-7
Positive Control 3: Jurkat
Positive Control 4: HepG2
Positive Control 5: U-87MG
Positive Control 6: Mouse kidney
Molecular Weight: Observed: 51kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: IDH2 Antibody: IDH, IDP, IDHM, IDPM, ICD-M, D2HGA2, mNADP-IDHICD-M, IDH
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+) . Five isocitrate dehydrogenases have been reported: three NAD (+) -dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+) -dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP (+) -dependent isozyme is a homodimer. The protein encoded by this gene is the NADP (+) -dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants.