Product Description
PC Antibody | 22-111 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 879-1178 of human PC (NP_001035806.1) .
Research Area: Cancer, Signal Transduction
Tested Application: WB, IF
Application: WB: 1:500 - 1:2000
IF: 1:10 - 1:100
Specificiy: N/A
Positive Control 1: HepG2
Positive Control 2: HeLa
Positive Control 3: MCF7
Positive Control 4: A-549
Positive Control 5: U-251MG
Positive Control 6: Mouse liver
Molecular Weight: Observed: 129kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: PC, pyruvate carboxylase, PCB
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.