Product Description
RFC4 Antibody | 19-694 | ProSci
Host: Rabbit
Reactivity: Human, Rat
Homology: N/A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 204-363 of human RFC4 (NP_002907.1) .
Research Area: Other
Tested Application: WB, IHC, IF, IP
Application: WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
IP: 1:50 - 1:200
Specificiy: N/A
Positive Control 1: HeLa
Positive Control 2: SW480
Positive Control 3: 22Rv1
Positive Control 4: SW620
Positive Control 5: K-562
Positive Control 6: HepG2
Molecular Weight: Observed: 40kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: RFC4, A1, MGC27291, RFC37
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) . RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.