Product Description
RPS6 Antibody | 19-991 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPS6 (NP_001001.2) .
Research Area: Signal Transduction
Tested Application: WB
Application: WB: 1:500 - 1:1000
Specificiy: N/A
Positive Control 1: HT-29
Positive Control 2: A-431
Positive Control 3: HeLa
Positive Control 4: NIH/3T3
Positive Control 5: Mouse liver
Positive Control 6: Rat liver
Molecular Weight: Observed: 35kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: RPS6, 40S ribosomal protein S6, S6, Ribosomal protein S6, Phosphoprotein NP33
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.