Product Description
TAF12 Antibody | 19-652 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human TAF12 (NP_001128690.1) .
Research Area: Other
Tested Application: WB
Application: WB: 1:500 - 1:2000
Specificiy: N/A
Positive Control 1: A-549
Positive Control 2: LO2
Positive Control 3: 293T
Positive Control 4: Raji
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Observed: 20kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: TAF2J, TAFII2transcription initiation factor TFIID subunit 12, TAF12 RNA polymerase II, TATA box binding protein (TBP) -associated factor, 20kDa, TAFII-20/TAFII-15, TAFII20/TAFII15, TATA box binding protein (TBP) -associated factor, RNA polymerase II, J, 20kD, transcription initiation factor TFIID 20/15 kDa subunits
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene.