Product Description
TAF5 Antibody | 22-793 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 656-800 of human TAF5 (NP_008882.2) .
Research Area: Other
Tested Application: WB, IF
Application: WB: 1:500 - 1:2000
IF: 1:50 - 1:100
Specificiy: N/A
Positive Control 1: Mouse brain
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Observed: 100kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: TAF (II) 10TAF2D, TAFII-10TAFII10transcription initiation factor TFIID subunit 5, TAF5 RNA polymerase II, TATA box binding protein (TBP) -associated factor, 100kDa, TATA box binding protein (TBP) -associated factor 2D, TATA box binding protein (TBP) -associated factor, RNA polymerase II, D, 100kD, transcription initiation factor TFIID 100 kD subunit, transcription initiation factor TFIID 100 kDa subunit
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID) , which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes an integral subunit of TFIID associated with all transcriptionally competent forms of that complex. This subunit interacts strongly with two TFIID subunits that show similarity to histones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex. Alternative splicing results in multiple transcript variants.