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Product Description
Goat Anti-Rabbit IgG (H&L) Antibody Pre-Adsorbed | XR-9900 | ProSci
Host: Goat
Reactivity: Rabbit
Immunogen: Rabbit IgG - whole molecule
Tested Application: E, WB, IHC
Application: Unconjugated Affinity Purified anti-Rabbit IgG is suitable for immunoblotting (western or dot blot) , ELISA and immunohistochemistry. Recommended dilutions are as follows, ELISA 1:35,000 - 1:325,000, Western Blot 1:3,000 - 1:35,000, Immunohistochemistry 1:1,000 - 1:5,000. (Optimal dilution should be determined by user) .
Specificiy: N/A
Purification: Unconjugated Affinity Purified anti-Rabbit IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins. Specificity was confirmed by ELISA at less than 0.5%
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% (w/v) Sodium Azide; pH 7.2
Concentration: 2.0 mg/mL
Storage Condition: Unconjugated Affinity Purified anti-Rabbit IgG can be stored vial at 4 ˚C prior to restoration. For extended storage aliquot contents and freeze at -20 ˚C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 ˚C as an undiluted liquid. Dilute only prior to immediate use.
Target Species: Reacts with the heavy chain of rabbit IgG.
