Product Description
Caspase-13 Antibody | 2245 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen: Caspase-13 antibody was raised against a 15 amino acid peptide near the amino terminus of bovine Caspase-13.
The immunogen is located within amino acids 80 - 130 of Caspase-13.
Research Area: Apoptosis, Cancer
Tested Application: E, WB, IHC-P
Application: Caspase-13 antibody can be used for detection of caspase-13 by Western blot at 1 μg/mL. A 43 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human, mouse, and rat samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1209 - HL60 Cell Lysate
Positive Control 2: Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive Control 3: Cat. No. 1463 - Rat Brain Tissue Lysate
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 43 kDa
Validation: N/A
Isoform: N/A
Purification: Caspase-13 Antibody is Ion exchange chromatography purified.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Caspase-13 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/ml
Storage Condition: Caspase-13 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: Caspase-13 Antibody: CASP13, Caspase-13, Evolutionary related interleukin-1-beta-converting enzyme, CASP-13
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Caspase-13 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death is finally caused by members of the caspase family of proteases and caspase activated DNases. A novel member in the caspase family was recently identified and designated ERICE (for Evolutionarily Related Interleukin-1 beta Converting Enzyme) and caspase-13. Caspase-13 belongs to the ICE subfamily of caspases. Overexpression of caspase-13 induces apoptosis. Caspase-13 was activated by caspase-8, which is a key enzyme in death receptor induced apoptosis. Caspase-13 is expressed in a variety of human tissues and cell lines.