Product Description
E2F1 Antibody | 18-476 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: A synthetic peptide of human E2F1
Research Area: Apoptosis, Cancer, Cell Cycle, Signal Transduction, Transcription
Tested Application: WB, IF
Application: WB: 1:500 - 1:1000
IF: 1:20 - 1:100
Specificiy: N/A
Positive Control 1: A-431
Positive Control 2: BxPC-3
Positive Control 3: U-87MG
Positive Control 4: NIH/3T3
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Observed: 70kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: E2F1, E2F-1, RBBP3, RBP3, RBAP1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP) , a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.