Product Description
Livin Antibody | 2505 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: Livin antibody was raised against a 17 amino acid peptide near the carboxy terminus of human Livin.
The immunogen is located within the last 50 amino acids of Livin.
Research Area: Apoptosis, Cancer
Tested Application: E, WB, IHC-P, IF
Application: Livin antibody can be used for detection of Livin by Western blot at 0.5 μg/mL. A band at 33 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: A lower but much weaker band at 30 kDa was detected in Raji cell lysate, which may represent the short form of Livin.
Positive Control 1: Cat. No. 1207 - Raji Cell Lysate
Positive Control 2: Cat. No. 1308 - Human Small Intestine Tissue Lysate
Positive Control 3: Cat. No. 11-801 - Human Small Intestine Tissue Slide
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 33 kDa
Validation: N/A
Isoform: N/A
Purification: Livin Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Livin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/ml
Storage Condition: Livin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: Livin Antibody: KIAP, LIVIN, MLIAP, RNF50, ML-IAP, KIAP, UNQ5800/PRO19607/PRO21344, Baculoviral IAP repeat-containing protein 7, Kidney inhibitor of apoptosis protein
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Livin Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel member in the IAP protein family was recently identified and designated Livin and KIAP for kidney IAP. Livin/XIAP contains a single baculoviral IAP repeat (BIR) domain and a RING finger domain and has two isoforms termed Livin-alpha and Livin-beta. Transfection of Livin in cells resulted in protection from apoptosis induced by FADD, BAX, RIP, RIP3 and DR6. Livin has direct interaction with several caspases including caspase-3, -7, and -9. Livin inhibits the activation of caspase-9 induced by Apaf-1, cytochrome c, and dATP. The two isoforms of Livin appear to have different functions and tissue distributions.