Product Description
NINJ1 Antibody | 7967 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: NINJ1 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human NINJ1.
The immunogen is located within the last 50 amino acids of NINJ1.
Research Area: Neuroscience, Apoptosis
Tested Application: E, WB, IHC-P, IF
Application: NINJ1 antibody can be used for detection of NINJ1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificiy: NINJ1 antibody is human, mouse and rat reactive.
Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 17 kDa
Observed: 16 kDa
Validation: N/A
Isoform: N/A
Purification: NINJ1 antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: NINJ1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: NINJ1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: NINJ1 Antibody: NIN1, NINJURIN, Ninjurin-1, Nerve injury-induced protein 1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Ninjurin-1 (NINJ1) is a homophilic adhesion molecule that is strongly induced in dorsal root ganglion (DRG) and Schwann cells following nerve injury and promotes neurite outgrowth of DRG neurons (1, 2) , suggesting that NINJ1 may play a role in nerve regeneration. NINJ1 is transcriptionally regulated by p53 and is induced by DNA damage in a p53-dependent manner. A deficiency of NINJ1 increases p53 expression, suppresses cell proliferation, but enhances apoptosis and premature senescence in a p53-dependent manner as well as radiation-induced cell mortality (3) .