Product Description
RELA Antibody | 31-180 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RELA.
Research Area: Transcription, Cancer, Signal Transduction, Immunology, Apoptosis,
Tested Application: E, WB
Application: RELA antibody can be used for detection of RELA by ELISA at 1:62500. RELA antibody can be used for detection of RELA by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 60 kDa
Validation: N/A
Isoform: N/A
Purification: Antibody is purified by Protein A/G affinity chromatography method.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration: batch dependent
Storage Condition: For short periods of storage (days) store at 4˚C. For longer periods of storage, store RELA antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Alternate Name: RELA, p65, NFKB3
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: p65 is a subunit of the nuclear factor kappa-B, a second messenger, which activates the transcription of a number of genes in multiple tissues. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex.