Product Description
TOP2A Antibody | 13-266 | ProSci
Host: Rabbit
Reactivity: Human, Mouse
Homology: N/A
Immunogen: A synthetic peptide of human TOP2A
Research Area: Apoptosis, Cancer, Cell Cycle
Tested Application: WB, IHC, IF
Application: WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:20 - 1:50
Specificiy: N/A
Positive Control 1: Jurkat
Positive Control 2: K-562
Positive Control 3: A375
Positive Control 4: SKOV3
Positive Control 5: NIH/3T3
Positive Control 6: N/A
Molecular Weight: Observed: 200kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, pH7.3.
Concentration: N/A
Storage Condition: Store at 4˚C. Avoid freeze / thaw cycles.
Alternate Name: TOP2A, TOP2, TP2A
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.