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Product Description
ZIPK Antibody | 2067 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: ZIP kinase antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase.
The immunogen is located within amino acids 270 - 320 of ZIPK.
Research Area: Apoptosis, Autophagy, Cancer
Tested Application: E, WB, ICC, IF
Application: ZIPK antibody can be used for detection of DNase II expression by Western blot at 1 μg/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2: Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 3: Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 4: Cat. No. 1208 - THP-1 Cell Lysate
Positive Control 5: Cat. No. 17-005 - Jurkat Cell Slide
Positive Control 6: N/A
Molecular Weight: Predicted: 26 kDa
Observed: 27 kDa
Validation: N/A
Isoform: N/A
Purification: ZIPK Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: ZIPK Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/ml
Storage Condition: ZIPK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: ZIPK Antibody: ZIP, ZIPK, Death-associated protein kinase 3, DAP-like kinase, DAP kinase 3
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: ZIPK Antibody: Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase. ZIP kinase contains an N-terminal kinase domain and a C-terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREB family. ZIP kinase has high sequence homology to DAP kinase (death-associated protein kinase) , which is a mediator of apoptosis induced by gamma interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues.
![ZIPK (IN) Antibody [Polyclonal] | PC-034](https://cdn11.bigcommerce.com/s-452hpg8iuh/images/stencil/500x659/products/391292/569755/klogo__76714.1643661101__50139.1644059240.png?c=2 "ZIPK (IN) Antibody [Polyclonal] | PC-034")
