Product Description
ACLY Antibody [E2B1] | 13-913 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Recombinant protein of human ACLY
Research Area: Cancer, Signal Transduction
Tested Application: WB, IHC, IF, IP
Application: WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
Specificiy: N/A
Positive Control 1: MCF7
Positive Control 2: Mouse colon
Positive Control 3: Mouse kidney
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Observed: 95kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Monoclonal
Clone: [E2B1]
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: ACL, ATPCL, CLATP, ATP-citrate synthase, ATP-citrate (pro-S-) -lyase, citrate cleavage enzyme
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440, 000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.