Product Description
ATF1 Antibody | 27-324 | ProSci
Host: Rabbit
Reactivity: Human, Rat
Homology: N/A
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATF1.
Research Area: Transcription, Cancer
Tested Application: E, WB
Application: ATF1 antibody can be used for detection of ATF1 by ELISA at 1:62500. ATF1 antibody can be used for detection of ATF1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.
Specificiy: N/A
Positive Control 1: 721_B Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 29 kDa
Validation: N/A
Isoform: N/A
Purification: Antibody is purified by peptide affinity chromatography method.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration: batch dependent
Storage Condition: For short periods of storage (days) store at 4˚C. For longer periods of storage, store ATF1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Alternate Name: ATF1, EWS-ATF1, FUS/ATF-1, TREB36
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: ATF1 binds the cAMP response element (CRE) (consensus: 5'-GTGACGT [AC] [AG]-3') , a sequence present in many viral and cellular promoters. ATF1 binds to the Tax-responsive element (TRE) of HTLV-I. ATF1 mediates PKA-induced stimulation of CRE-reporter genes.