Product Description
Cyclin D1 Antibody [G9H10] | 13-786 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: Recombinant protein of human Cyclin D1
Research Area: Cancer, Cell Cycle, Signal Transduction
Tested Application: WB, IHC, IF, IP
Application: WB: 1:1000 - 1:5000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
IP: 1:20 - 1:50
Specificiy: N/A
Positive Control 1: HeLa
Positive Control 2: PC-12
Positive Control 3: SH-SY5Y
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Observed: 34kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Monoclonal
Clone: [G9H10]
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene, CCND1, BCL1, PRAD1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.