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Product Description
NCF4 Antibody | 13-218 | ProSci
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: A synthetic peptide of human NCF4
Research Area: Cancer, Cell Cycle, Signal Transduction
Tested Application: WB, IHC, Flow
Application: WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
Flow: 1:20 - 1:50
Specificiy: N/A
Positive Control 1: K-562
Positive Control 2: Raji
Positive Control 3: HL-60
Positive Control 4: LO2
Positive Control 5: MCF7
Positive Control 6: Mouse lung
Molecular Weight: Observed: 39kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, pH7.3.
Concentration: N/A
Storage Condition: Store at 4˚C. Avoid freeze / thaw cycles.
Alternate Name: NCF4, MGC3810, NCF, P40PHOX, SH3PXD4
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox) , which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI (3) kinase, which suggests its role in PI (3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
