Product Description
RB Antibody | 13-425 | ProSci
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: A synthetic peptide of human RB1
Research Area: Cancer, Cell Cycle, Transcription
Tested Application: WB, IHC, Flow
Application: WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
Flow: 1:20 - 1:50
Specificiy: N/A
Positive Control 1: HeLa
Positive Control 2: Jurkat
Positive Control 3: SW480
Positive Control 4: HT-1080
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Observed: 120kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, pH7.3.
Concentration: N/A
Storage Condition: Store at 4˚C. Avoid freeze / thaw cycles.
Alternate Name: RB1, RB, pRb, OSRC, pp110, p105-Rb
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB) , bladder cancer, and osteogenic sarcoma.